International Journal of Morphology
versión On-line ISSN 0717-9502
BECERRA G, Daniela et al. Evaluation of Mesenchymal Stem Cell Proliferation Using Different Low Intensity Pulsed Ultrasound Intensities. Int. J. Morphol. [online]. 2012, vol.30, n.2, pp.621-626. ISSN 0717-9502. http://dx.doi.org/10.4067/S0717-95022012000200043.
Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.
Palabras clave : Adult stem cells; Mesenchymal stem cells; ultrasound.