International Journal of Morphology
versión ISSN 0717-9502
CORNEJO URIBE, Ricardo et al. Morphometric Study of Effect 16 J/cm2 Infrared Laser in Rat Hepatocytes. Int. J. Morphol. [online]. 2011, vol.29, n.2, pp. 650-655. ISSN 0717-9502. doi: 10.4067/S0717-95022011000200058.
Sprague Dawley rat livers were irradiated with 8 and 16 J/cm2 daily doses during 15 days from the AsGA laser, equivalent to 904 nm. Liver samples were surgically removed from rats previously anesthetized, then processed for electronic microscopic transmission and subsequently studied and subjected to morphometric techniques using 8.500 X enlargements with special emphasis on quantifying cellular component volumetric fractions. The result analysis of the hepatocytes irradiated with 8 and 16J/cm2 doses revealed that there were marked differences between the volumetric fractions of cellular components that determine cellular functionality and protein synthesis, a quantification that clearly demonstrates that with a dose of 8 J/cm2, the optimum stimuli is achieved in order to obtain high functionality, while with a dose of 16 J/cm2 a notable reduction of that functionality is noted, concluding that the effects of specific infrared radiation doses provoke a drastic transformation of its components in hepatocytes, and therefore its functionality, activating or inhibiting it, and representing the effect of these stimulations over this elevated metabolism cellular type.
Palabras clave : Infrared laser; Hepatocyte; Morphometry.