International Journal of Morphology
versión On-line ISSN 0717-9502
MARTINEZ RIVERA, Pablo; PINA OSUNA, Ana Karina; SOTO DOMINGUEZ, Adolfo y GARCIA GARZA, Rubén. Histological and Immunohistochemical Comparison of Tissue Samples Processed by the Conventional Technique or the Acetone Simplified Method. Int. J. Morphol. [online]. 2011, vol.29, n.2, pp.575-580. ISSN 0717-9502. http://dx.doi.org/10.4067/S0717-95022011000200046.
The conventional technique (CT) using alcohol as a drying agent, is a useful method of histology and pathology in the laboratory. Recently, a new simplified method with acetone (TA) was designed in an attempt to reduce the processing time of the tissues (12 to 4 hours), the cost of reagents, and as an important factor to have an accurate and prompt diagnosis . This study compared the histological and immunohistochemical parameters in breast cancer, colon and kidney specimens treated with both methods. The sections were stained with H & E or Masson trichrome. Immunohistochemistry study with antibodies specific for the identification of cytokeratin AE1/AE3, the estrogen receptor and progesterone receptor was also carried out. Blind study was conducted by three specialists in morphology, who evaluated the nuclear staining, staining of the cytoplasm and friability or changes in tissue structure. The specificity and sensitivity of antibody binding were also evaluated. The values obtained for each parameter were statistically analyzed with Student t test. Our results show that the methods of CT and TA did not alter histological features as the pattern of staining, and tissue changes were detected. Immunohistochemical positivity was similar for both methods. There was no statistical difference between TC and TA. Our results suggest that implementation of the TA did not alter the histological and immunohistochemical properties and could be a useful method for morphological analysis.
Palabras clave : Alcohol; Acetone; Histological processing; Immunohistochemistry.