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International Journal of Morphology
versión On-line ISSN 0717-9502
VASCONCELLOS C, Adriana et al. Analysis of the Expression of Estrogen Receptors in the Endometrium of Ewes Fed with Normal and Supplementary Diet. Int. J. Morphol. [online]. 2009, vol.27, n.4, pp.1093-1098. ISSN 0717-9502. http://dx.doi.org/10.4067/S0717-95022009000400023.
The effects of nutrition on sheep reproduction have been studied by many authors. The interest has focused on the effects of diet quality in sheepin in which low plans n have low plane nutrition can cause abnormalities of the ovum or the embryo, luteal inadequancy and failure of the supply of progesterone to the uterus or failurein the mechanisms involved maternal of pregnancy. There are papers about the relation food-hormones, however, the existing information on endometrial steroid hormone receptors, which are essential for them to act, is very scarce. The aim of this study was to comparatively analyze the expression of estrogen receptor (ER) in endometrial cycle of sheep fed diet supplemented and normal diet. The expression of the receptor protein and the transcript were detected by immunohistochemical analysis and RT real-time PCR, respectively. The results showed immunohistochemical expression of RE in glandular and carunculares areas, especially in an intense inmunorreacción stromal cell nuclei and glandular epithelium. Detected increased expression of transcripts of RE in sheep fed a supplement with respect to not receiving a dietary supplement. We discuss the possible use of this information for application in breeding sheeps programs.
Palabras clave : Sheep; Endometrial; Estrogen receptor; Progesterone receptor; Nutrition.