International Journal of Morphology
On-line version ISSN 0717-9502
SUAZO GALDAMES, Iván Claudio and ROA HENRIQUEZ, Ignacio Javier. Microscopio Anatomy of the Salivary Glands for Mean of Conventional and not-Conventional Histologic Technique. Int. J. Morphol. [online]. 2008, vol.26, n.3, pp. 689-695. ISSN 0717-9502. http://dx.doi.org/10.4067/S0717-95022008000300029.
The techniques of fixation and conservation allow to stop the processes of tissues disorganization and they are necessary to analyze the microscopic anatomy of them. The purpose of this study was to analyze the histologic characteristic of the parotid and submandibular glands obtained from three human cadavers fixed and conserved by means of: a) conservative solution based on formaldehyde (Sample I) and b) camera of cold for 12 hours (Sample II), both samples processed for hematoxilin-eosin (H-E); c) plastination with epoxic resin (Sample III) and processed for H-E and with methylene blue - eosin without previous inclusión. The characteristics of the adenomer and glandular ducts system were analyzed. The best characteristics were in the sample II, with a good detail level in the glandular parenchyma, a greater basophilia was presented in the sample I. The sample III it presented a low detail level to the microscopic observation, the best results were obtained using methylene blue. The biggest difficulties in the histologic process of the plastinated specimens were in the cut and time for tintion. The results suggest that it is possible to obtain histologic preparations from autopsies in fixed cadavers and conserved for teaching and anatomical investigation.
Keywords : Histologic technique; Plastination; fixation; Conservation; Salivary glands.