International Journal of Morphology
On-line version ISSN 0717-9502
MARCAL NATALI, Maria Raquel; HUBNER DE MIRANDA-NETO, Marcílio and ORSI, Antônio Marcos. MORPHOMETRY AND QUANTIFICATION OF THE MYENTERIC NEURONS OF THE DUODENUM OF ADULT RATS FED WITH HYPOPROTEIC CHOW. Int. J. Morphol. [online]. 2003, vol.21, n.4, pp. 273-277. ISSN 0717-9502. doi: 10.4067/S0717-95022003000400003.
Morphometric and quantitative analyses were carried out on the myenteric neurons of the duodenum, to study possible alterations resulting from a supply of low-protein level chow to adult Wistar rats. The animals were divided in two groups: nourished (N) fed with ration containing 22% protein for 210 days and the undernourished (D) fed with ration containing 8% protein from the 90th day of age on and during the next 120 days. Whole-mounts were stainedby the method of Giemsa and the histochemical reaction of NADH-diaphorase enzyme, to estimate the neuronal population, which revealed a greater neuronal density in undernourished rats, regardless of the technique employed. The morphometric studies of the cell body profiles of 500 neurons of each group indicated a reduction on the cell body size and an increase on the proportion of small neurons on the rats subjected to the hypoproteic chow
Keywords : Proteic deficiency; Duodenum; GIEMSA; Myenteric neurons; NADH-diaphorase; Rats.