Abstract

CORNEJO U, Ricardo. MORFOMETRÍA DEL NUCLEOLO EN LA DIFERENCIACIÓN CELULAR DEL EPITELIO MAMARIO. Rev. chil. obstet. ginecol. [online]. 2010, vol.75, n.2, pp. 107-111. ISSN 0717-7526.  http://dx.doi.org/10.4067/S0717-75262010000200006.

Background: The biological process of cell differentiation is the traslation of multiple nuclear and cytoplas-mic processes that determine complex and fundamental changes in ultrastructure, biochemistry and cell physiology, which can be quantifed using morphometric techniques. Objective: To show in quantitative and morphological terms changes experienced by the nucleolus belonging to HC11 line mammary cells both, in proliferating and differentiation process. Methods: A study at the level of transmission electron microscopy of cell types in stage of cell proliferation in comparison with stage of differentiation was designed to quantify variations of nucleolus and their relation to structures involved in protein synthesis. Results: Marked differences in the area, volume and length of the nucleolus were found between normal-proliferating cell types and those in mechanism of differentiation. Conclusion: The mammary cells in differentiation process show a dramatic decline in its nucleoli and their ribonucleoproteins generate basically ribosomes attached at endo-plasmic reticulum, synthesizing export proteins.

Keywords : Cellular diffentiation; morphometry; nucleolus; mammary glands.