Abstract

CORNEJO U, Ricardo. ANÁLISIS MORFOMÉTRICO DE LISOSOMAS: MARCADORES DE TRANSFORMACIÓN CELULAR DEL EPITELIO MAMARIO. Rev. chil. obstet. ginecol. [online]. 2009, vol.74, n.2, pp. 83-87. ISSN 0717-7526.  doi: 10.4067/S0717-75262009000200003.

Background: Cellular transformation is the result mechanism of powerful action generated by transforming oncogene over a normal cell, which with the subsequent oncoprotein expression leads to drastic changes in morphology as well as in cell components volumes, generating a cell with a different function. Objective: To specify the modifications that characterizes the transforming mechanism in mammary epithelial cells transfected with the ras oncogene comparing them with its normal cell type. Method: Transmission electronic microscopy using morphometric techniques was applied to this cell types, emphasizing lysosomes variations, trying to clarify its role in each cell type metabolism. Results: Everyone lysosomal parameters examined in transformed cell type present significant differences regarding to the normal cells. Conclusion: The drastic changes in lysosomes reflected in the acquisition of new energy requirements and metabolism in the transformed cell.

Keywords : Cellular transformation; lysosomes; morphometry.