versión On-line ISSN 0717-6538
ALAYO, Marianella y IANNACONE, José. ECOTOXICOLOGICAL ASSAY WITH CRUDE OIL, DIESEL 2 AND DIESEL 6 WITH TWO SUBSPECIES OF BRACHIONUS PLICATILIS MÜLLER, 1786 (ROTIFERA: MONOGONONTA). Gayana (Concepc.) [online]. 2002, vol.66, n.1, pp. 45-58. ISSN 0717-6538. http://dx.doi.org/10.4067/S0717-65382002000100007.
ABSTRACT We conducted standardized ecotoxicological assay with the rotifer Brachionus plicatilis rotundiformis line S and B. plicatilis hepatotomus line L took as tools to evaluate aquatic environment, employing the Water-Soluble Fraction (WSF) of three hydrocarbons: Crude oil (CO), Diesel 2 (D2) and Diesel 6 (D6). The values determined of LC50 for B. plicatilis rotundiformis at 24 and 48 h exposure of WSF from CO, D2 and D6 were 0,13, 0,04; 0,65, 0,14 and 4,20, 0,33 mg/L, respectively. The values determined of CL50 for B. plicatilis hepatotomus at 24 and 48 h exposure of WSF from CO, D2 and D6 were 0,23, 0,05; 1,36, 0,13 and 3,47, 1,01 mg/L, respectively. Among the three hydrocarbons evaluated the order of ecotoxicity decreased at 24 and 48 h exposure for both subspecies were: Crude oils > Diesel 2 > Diesel 6. We did not find differences between the sensibility of both subspecies of Brachionus. We proposed a protocol for the bioassay employing B. plicatilis rotundiformis and B. plicatilis hepatotomus as a tool to evaluate aquatic environment. This specie shows the next advantages: wide distribution in marine environment, trustworthily, repeatedly, short last of the bioassay and high sensibility when we compared microcrustaceans as nauplii of Artemia salina and zoeas of Emerita analoga
Palabras clave : Ecotoxicity; rotifer; Brachionus; petrol; bioassays; water- soluble fraction.