Electronic Journal of Biotechnology
On-line version ISSN 0717-3458
RAMIREZ, Ingrid; DORTA, Fernando; CUADROS-INOSTROZA, Álvaro and PENA-CORTES, Hugo. Callus induction and plant regeneration of Ulex europaeus. Electron. J. Biotechnol. [online]. 2012, vol.15, n.4, pp. 7-7. ISSN 0717-3458.
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.
Keywords : antioxidants; callus induction; Fabaceae; gorse; plant regeneration; Ulex europaeus.