Electronic Journal of Biotechnology
version ISSN 0717-3458
WANG, Chuan Tang et al. Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut. Electron. J. Biotechnol. [online]. 2010, vol.13, n.5, pp. 18-19. ISSN 0717-3458.
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.
Keywords : identification; NIRS; PCR; peanut; true hybrid.