Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
ACEVEDO, Cristian et al. Measuring b-Galactosidase activity at pH 6 with a differential pH sensor. Electron. J. Biotechnol. [online]. 2009, vol.12, n.2, pp. 12-13. ISSN 0717-3458.
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
Palabras llave : b-Galactosidase; delta milli pH; differential pH.