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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


ACEVEDO, Cristian et al. Measuring b-Galactosidase activity at pH 6 with a differential pH sensor. Electron. J. Biotechnol. [online]. 2009, vol.12, n.2, pp. 12-13. ISSN 0717-3458.

The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Palabras clave : b-Galactosidase; delta milli pH; differential pH.

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