Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
CHIBEU, Andrew et al. Genetic and physical map of broad host range cosmid pRG930cm. Electron. J. Biotechnol. [online]. 2009, vol.12, n.2, pp. 10-11. ISSN 0717-3458.
We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.
Palabras clave : cosmid; pRG930cm; Pseudomonas; sequencing.