Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
WANG, Chuan Tang et al. A rapid and cheap protocol for preparation of PCR templates in peanut. Electron. J. Biotechnol. [online]. 2009, vol.12, n.2, pp. 9-10. ISSN 0717-3458. http://dx.doi.org/10.4067/S0717-34582009000200009.
This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.
Palabras clave : DNA extraction; groundnut; PCR; peanut.