Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
PINTATHONG, Punyawatt; RICHARDSON, David J; SPIRO, Stephen y CHOORIT, Wanna. Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond. Electron. J. Biotechnol. [online]. 2009, vol.12, n.2, pp. 4-5. ISSN 0717-3458. http://dx.doi.org/10.4067/S0717-34582009000200004.
The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accumulation. Enzymatic studies revealed that the membrane-bound nitrate reductase and the periplasmic nitrite reductase had activities of 998 ± 28 and 373 ± 18 nmol (mg protein)-1 min-1, respectively after growing Paracoccus pantotrophus P16 in medium supplemented with 1.5 µM Fe3+. If provided with 1.5 µM Fe3+and 2.4 µM Mo6+, the membrane-bound nitrate reductase activity increased to 6,223 ± 502 nmol (mg protein)-1 min-1 and the periplasmic nitrite reductase was 344 ± 20 nmol (mg protein)-1 min-1. The results indicated that an addition of Fe3+ and Mo6+ led to an overstimulation of nitrate reductase activity as compared with nitrite reductase activity. When glucose was supplied, the minimal ratio of carbon per nitrate (C/N) was 2.31 mg C/mg NO3--N with denitrification yield of 0.45 g NO3--N/g C. Addition of ethanol instead of glucose, the minimal ratio of C/N was 1.15 mg C/mg NO3--N with denitrification yield of 1.08 g NO3--N/g C.
Palabras clave : metal ions; nitrate reductase; nitrite accumulation; nitrite reductase; Paracoccus pantotrophus.