Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
NATARAJAN, Chandrasekhar et al. Universal protocol for generating 100bp size standard for endless usage. Electron. J. Biotechnol. [online]. 2008, vol.11, n.2, pp. 126-129. ISSN 0717-3458.
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
Palabras llave : 100bp ladder; cost effective; DNA size standards; endless usage.