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Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458

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NATARAJAN, Chandrasekhar et al. Universal protocol for generating 100bp size standard for endless usage. Electron. J. Biotechnol. [online]. 2008, vol.11, n.2, pp. 126-129. ISSN 0717-3458.

Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.

Palabras llave : 100bp ladder; cost effective; DNA size standards; endless usage.

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