Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
TANG WANG, Chuan et al. Isolation of simple sequence repeats from groundnut. Electron. J. Biotechnol. [online]. 2007, vol.10, n.3, pp.473-480. ISSN 0717-3458. http://dx.doi.org/10.4067/S0717-34582007000300015.
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
Palabras clave : groundnut; isolation; simple sequence repeat.