Electronic Journal of Biotechnology
On-line version ISSN 0717-3458
ASFORA SARUBBO, Leonie; MOURA DE LUNA, Juliana and DE CAMPOS-TAKAKI, Galba Maria. Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002. Electron. J. Biotechnol. [online]. 2006, vol.9, n.4. ISSN 0717-3458. http://dx.doi.org/10.4067/S0717-34582006000400008.
Evaluation of both tenso-active and emulsifying activities indicated that a biosurfactant was produced by the newly isolated and promising strain Candida glabrata isolated from mangrove sediments. The extracellular water-soluble emulsifying agent was isolated and identified as a heteropolymer. The maximum of bioemulsifier production was observed when the strain was grown on soluble and insoluble substrates cotton seed oil plus glucose, reaching values of 10.0 g/l after 144 hrs at 200 rpm. The cell-free culture broth containing the examined agent lowered the surface tension of the medium to 31 mN/m. Stable and compact emulsions with emulsifying activity of 75% of cotton seed oil were detected. The emulsification capacity remained practically unaltered within a wide pH (2-12), temperature (4-80ºC) ranges and under NaCl concentrations up to 10%.
Keywords : biosurfactant; Candida; emulsifier; fermentation; glucose; vegetal oil.