SciELO - Scientific Electronic Library Online

 
vol.9 issue4Modulation of unsaturated fatty acids content in algae Spirulina platensis and Chlorella minutissima in response to herbicide SAN 9785Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Electronic Journal of Biotechnology

On-line version ISSN 0717-3458

Abstract

PEREIRA DUTA, Flávia; PESSOA DE FRANCA, Francisca  and  DE ALMEIDA LOPES, Léa Maria. Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method . Electron. J. Biotechnol. [online]. 2006, vol.9, n.4, pp. 0-0. ISSN 0717-3458.  http://dx.doi.org/10.4067/S0717-34582006000400007.

The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 ± 1)ºC] and pH value (7.0 ± 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 23 factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded YP/S (g/g) = 0.35.

Keywords : exopolysaccharides; experimental design; response surface method; Rhizobium sp..

        · text in English     · pdf in English