Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
MARTINEZ, Carolina; PETRUCCELLI, Silvana; GIULIETTI, Ana María y ALVAREZ, María Alejandra. Expression of the antibody 14D9 in Nicotiana tabacum hairy roots. Electron. J. Biotechnol. [online]. 2005, vol.8, n.2, pp. 51-57. ISSN 0717-3458. http://dx.doi.org/10.4067/S0717-34582005000200006.
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
Palabras clave : catalytic antibodies; in vitro cultures; molecular farming; recombinant antibodies; tobacco.