Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
TRUJILLO TOLEDO, Luis Enrique et al. Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast. Electron. J. Biotechnol. [online]. 2004, vol.7, n.2, pp. 115-123. ISSN 0717-3458.
The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures
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