Electronic Journal of Biotechnology
versión On-line ISSN 0717-3458
LEON FISZMAN, Gabriel; KARARA, Armando Luis; FINOCCHIARO, Liliana María Elena y GLIKIN, Gerardo Claudio. A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads. Electron. J. Biotechnol. [online]. 2002, vol.5, n.3, pp. 23-24. ISSN 0717-3458.
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.
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