SciELO - Scientific Electronic Library Online

vol.3 número2An international political economic view of the biotechnology industrySemi-quantitative detection of genetically modified grains based on CaMV 35S promoter amplification índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Electronic Journal of Biotechnology

versión On-line ISSN 0717-3458


LEROY, Xavier J.; LEON, Karine  y  BRANCHARD, Michel. Plant genomic instability detected by microsatellite-primers. Electron. J. Biotechnol. [online]. 2000, vol.3, n.2, pp.5-10. ISSN 0717-3458.

This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture.

Palabras clave : .

        · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons