Revista chilena de infectología
versión impresa ISSN 0716-1018
LOPEZ, Javier et al. Morphological and molecular identification of canine filariae in a semi-rural district of the Metropolitan Region in Chile. Rev. chil. infectol. [online]. 2012, vol.29, n.3, pp. 248-289. ISSN 0716-1018. http://dx.doi.org/10.4067/S0716-10182012000300006.
Introduction: To date, there has been no definitive confirmation of the presence of zoonotic dirofilariasis in dogs in Chile. Objectives: To study the presence of dirofilarias in blood samples from dogs collected in a semi-rural district near Santiago and to compare their frequency in dogs with and without dermatological manifestations. Methods: We examined 100 blood samples for dog filariae infections using microscopic methods (modified Knott technique). 50 dogs presented dermatological symptoms or signs compatible with filarial infections and 50 were asymptomatic. ITS-2 and 12s rDNA gene amplification by PCR and sequencing were performed in samples microscopically positive for microfilariae. Results. We observed microfilariae in 22 dogs (22%). Of these, 16/50 (32%) were symptomatic and 6/50 (12%) were asymptomatic (p = 0.02). Morphologically, the majority of micro-filariae were similar to Dirofilaria repens, although many had a bigger size than previously described. Nucleotide sequencing of the amplified genes showed no more than 95% homology with the D. repens sequences available for comparison. D. reconditum and D. dracunculoides infections were also identified. Conclusions: These features might indicate the presence of new species of Dirofilaria or a D. repens close related variant in Chile.
Palabras clave : Canine filariasis; microfilariae; Dirofilaria repens; zoonotic dirofilariasis.