Parasitología al día
versión impresa ISSN 0716-0720
GORMAN, Texia; SANCHEZ, Roxana; FREDES, Fernando y ALCAINO, Hector. INMUNODIAGNOSTICO DE FASCIOLOSIS BOVINA MEDIANTE ELISA Y WESTERN BLOT. Parasitol. día [online]. 1998, vol.22, n.1-2, pp. 16-22. ISSN 0716-0720. http://dx.doi.org/10.4067/S0716-07201998000100003.
Immunodiagnosis of bovine fasciolosis was evaluated in terms of its sensitivity and specificity by ELISA and Western blot using a crude excretory-secretory preparation (C E-S) and an E-S partially purified chromatographic fraction of Fasciola hepatica (<30 kDa) as antigens. Serum samples from 52 bovines with natural fasciolosis, 18 without the infection and 48 bovines with hydatid cysts, all checked by post mortem examination, were tested as well. The sensitivity and specificity obtained by ELISA using the C E-S antigens were 53% and 100%, respectively. Likewise, when using the partially purified fraction, the sensitivity of ELISA test increased to 90% with a 100% specificity. The C E-S antigens reacted specifically with sera from infected bovines exhibiting bands of 14, 22, 27-39 and 37-38 kDa in Western blots. When the partially purified fraction was tested, the 28-30 kDa band was consistently detected by sera from all infected bovines and was absent in sera from uninfected as well as in sera from those with hydatid infection. These results indicate that F. hepatica presents different antigens, among which the 28-30 kDa fraction represents polypeptides with diagnostic potential that can be further purified and applied in the immunodiagnosis of bovine fasciolosis corroborating previous results recorded in other animal species
Palabras clave : fasciolosis; bovine fasciolosis; immunodiagnosis; Fasciola hepatica; antigens.