Boletín de la Sociedad Chilena de Química
versión impresa ISSN 0366-1644
BUNSTER, Marta; CONTRERAS-MARTEL, Carlos; BRUNA, Carola y MARTINEZ- OYANEDE, José. R-FICOERITRINA DE GRACILARIA CHILENSIS: ESTABILIDAD E INTERACION ENTRE SUBUNIDADES. Bol. Soc. Chil. Quím. [online]. 2000, vol.45, n.2, pp. 303-309. ISSN 0366-1644. doi: 10.4067/S0366-16442000000200018.
Numerous biological macromolecules are arranged in complex interaction systems, to perform their biological function. Phycobiliproteins, the main polypeptidic components of the phycobilisomes, are very good examples for explaining these interactions because their light harvesting and conduction function depend strongly on the three dimensional arrangement of the complex. The functional unit of R-phycoerythrin from Gracilaria chilensis is (ab)6 and all the subunits require to be perflectly packed to perform the function. For this reason it was selected to study the rol of the different type of interactions in the stability of the protein. To distinguish the contribution of some of the different type of interactions , differential spectrocopy was used to follow the effect of temperature, ionic strengh and presence of urea and the structural information was used to analyse the interaction surfaces that are produced during the association. Our results suggest that ( ab ) is the minimum specie detectable because its interaction surface presents an important hydrophobic component and because in this process, 9 new hydrogen bonds are formed, that contribute to the stability. The stabilization of (ab)6, requires the contribution of all the subunits involved
Palabras clave : R-phycoerythrin; interaction surfaces; stability.