Archivos de medicina veterinaria
versión ISSN 0301-732X
SCHOFFER, JT et al. Application of the EROD-H4IIE bioassay for the determination of dioxins in broiler chicken meat: an equivalence study with high resolution gas chromatography coupled to high resolution mass spectrometry. Arch. med. vet. [online]. 2011, vol.43, n.3, pp. 259-266. ISSN 0301-732X. doi: 10.4067/S0301-732X2011000300008.
The EROD bioassay with H4IIE cell line was applied in this study to determine the equivalence of the results for chicken meat between the EROD-H4IIE Bioassay in pg TCDD-EQ/g of tissue, and the results of the gas chromatography coupled to high resolution spectrometry (HRGC/HRMS) in pg WHO-TEQ/g of fat of TEQs. 41 compound samples of chicken drumsticks were used. The samples were obtained in slaughtering plants of 4 different production facilities of Chile, during the sacrifice of animals (38 and 43 days old) between 2004 and 2007. Each sample was analysed with both analytical techniques. A regression model for the equivalence of both techniques was determined from the results. The model obtained was: HRGC/ HRMS = 0.481 + 0.051[EROD-H4IIE]2, R2 = 0.885, therefore for a value in EROD-H4IIE of 2.2 pg TCDD-EQ/g of tissue it is estimated that it will correspond to 0.73 pg WHO-TEQ/g of fat in HRGC/HRMS. Also, for the same value of EROD-H4IIE with a 95% confidence, an estimate as an upper limit equal to 0.83 pg WHO-TEQ/g of fat in HRGC/HRMS will be obtained. An upper limit equal to 0.88 pg WHO-TEQ/g of fat in HRGC/HRMS is estimated by taking the same value of EROD-H4IIE with 99% confidence. It is concluded that de EROD-H4IIE bioassay can be applied as a screening method in animal production systems and specifically in broiler chicken production.
Palabras clave : dioxin; H4IIE bioassay; HRGC/HRMS; EROD.