Archivos de medicina veterinaria
version ISSN 0301-732X
BERRIOS, O; VALDEBENITO, I; TREULEN, F and UBILLA, A. Cold storage of sperm of rainbow trout (oncorhynchus mykiss): Effect on motility, intracellular superoxide, plasma membrane integrity and mitochondrial membrane potential. Arch. med. vet. [online]. 2010, vol.42, n.3, pp. 179-186. ISSN 0301-732X. doi: 10.4067/S0301-732X2010000300009.
In teleostei, as opposed to what happens in mammals, most of the studies that evaluate the quality storages of semen are oriented toward the exposure of spermatozoa to some reactive oxygen species (ROS), the utilization of antioxidants in the diet, or the incorporation of these in seminal plasma. There is no available the literature covering the presence of superoxide ions (O2._), or the function of these on the interior of the spermatozoa that have been stored. In this study, we evaluated the effect of storage on intracellular O2._, motility, plasmatic membrane integrity, and mitochondrial membrane potential (ΔΨMit) of spermatozoa of rainbow trout (oncorhynchus mykiss). Semen was extracted and put into storage for 12 days at 4 ºC. Spermatozoa motility, (ΔΨMit), plasmatic membrane integrity were evaluated every 4 days, and intracellular O2._ was detected. It was found that 82.59% of the cells were positive for the staining of O2._ on the day of sample extraction, while sperm motility, ΔΨMit and plasmatic membrane integrity only showed deterioration after the eighth day of storage. Only ΔΨMit is correlated negatively with O2._ starting from the eighth day of storage (r = -0.56 P < 0.05). Regarding motility and plasmatic membrane integrity, these were not affected by intracellular O2._, although the deterioration observed in these last two indicators, could have been caused by the prolonged contact of the spermatozoa with contaminating agents that were not evaluated in this study.
Keywords : storage; intracellular superoxide; rainbow trout; spermatozoa.