Archivos de medicina veterinaria
versión impresa ISSN 0301-732X
LLAMBI, S y NUNEZ, R. Identification of chromosome fragility using 5azacytidine in cattle lymphocytes. Arch. med. vet. [online]. 2007, vol.39, n.1, pp. 63-66. ISSN 0301-732X. doi: 10.4067/S0301-732X2007000100009.
Common fragile sites are regions of the eukaryotic chromosome with a tendency to show breaks or gaps when inductors such as 5azacytidine are added to the lymphocyte culture media. Recently, chromosome fragile sites have been correlated with hereditary defects affecting humans and domestic animals (mental retardation syndrome, parakeratosis, baldy calf syndrome and low fertility). A spontaneous fragile site in the sexual chromosome X (FRA Xq 3.1) was observed in lymphocyte cultures of Uruguayan Holstein-Friesian cattle. In the present study, the chromosome fragility expression in lymphocyte cultures of Uruguayan Holstein-Friesian female cattle treated with 5azacytidine was analized. A number of different fragility regions were scored considering autosomes and sexual X chromosomes.
Palabras clave : 5azacytidine; fragile sites; chromosomes; cattle.