Archivos de medicina veterinaria
versión impresa ISSN 0301-732X
UZAL, F.A. et al. A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats. Arch. med. vet. [online]. 1999, vol.31, n.1, pp. 131-134. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X1999000100015.
A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later
Palabras clave : fluorescence polarization assay; goats; Clostridium perfringens; epsilon toxin antibodies.