Revista médica de Chile
versión ISSN 0034-9887
PEREZ N, Claudio; RABANALES T, Ramón; LARRONDO L, Milton y ALFARO L, Jorge. Frequency of minor histocompatibility antigens among Chilean blood donors. Rev. méd. Chile [online]. 2012, vol.140, n.5, pp. 555-560. ISSN 0034-9887. doi: 10.4067/S0034-98872012000500001.
Background: Minor histocompatibility antigens (mHAgs) play a critical role in the immune responses associated with allogeneic stem cell transplantation, such as graft versus host disease (GVHD) and graft-versus-tumor (GVT). Aim: To determine the gene frequencies of the mHAgs HA-1, HA-2 and HA-8 in Chilean Blood Bank donors. Material and Methods: Blood from 192 blood donors was analyzed. The presence of haplotype HLA-A*02 was determined by flow cytometry. The frequency of mHAgs was determined by allele specific polymerase chain reaction in genomic DNA. Results: Sixty one participants were carriers of the haplotype HLA-A*02. The relative allele frequency HA-1H was 45%, HA-Ir 55%, HA-2V 80.6%, HA-2M 19.4%, HA-8R 49.8% and HA-8P was 50.2%. Based on mHAgs disparity between HA-1, HA-2 or HA-8, the probability to generate a GVT response in HLA-A*02 individuals was 40%. Conclusions: The mHAgs frequency in Chilean population is under Hardy-Weinberg equilibrium and they are similar to those of other ethnic populations in the world.
Palabras clave : Blood donors; Haplotypes; HLA antigens.