Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No.1, Issue of April 15, 2005 
 

Table 1. Traditional functional genomics *.

Methods

Minimum Material

Throughput

Sequencing

Application

Sequencing base

 

 

 

 

EST

1-5 ug polyA RNA

High

High

gene expression

SAGE

1-5 ug polyA RNA

High

High

gene expression

Gel base

 

 

 

 

DD

10-100 ng polyA RNA

Medium

Low

Parallel gene expression

Colony base

 

 

 

 

Subtractive cloning

10-100 ng polyA RNA

Medium

Low

Parallel gene expression

Hybridization base

 

 

 

 

Microarray

>1 ug polyA RNA

High

N/A

Parallel gene expression

Protein base #

 

 

 

 

Two-D gel electrophoresis

106-107 cells

High

N/A

Parallel protein expression

Mass spectrometry

105-106 cells

High

N/A

Parallel protein expression

Functional array

 

 

 

 

Repressor system

N/A

N/A

Low

Protein-protein reaction

Yeast- library system

N/A

N/A

Medium

Protein


* Sample concentration: Claudio et al. 1998; Trendelenburg et al. 2002; Bosch et al. 2000; Carulli, et al. 1998; Oppermann et al. 2000.

# Some journals used as protein concentration


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